A number of churches and denominations took part in the service, which included readings, singings, a moment of silence and the National Anthem.
Reverend William Heenan, St Columba's Church, said: 'The sudden death of our Queen has been a shock and also a time of great sadness across the Western Isles, the Nation, the Commonwealth and beyond.
'She promised at her Coronation to serve for as long as she lived, and she certainly did that. Her unwavering commitment to her faith strengthened and guided her and she recognised her need of God’s help to fulfil her Calling.
'She has given a lifetime of unstinting service. Our new King Charles III, could have no better mentor than his Mother the Queen for the role he is now called to fulfil. Her life revolved around bringing people together from all walks of life and was also one of servant leadership.
'Our service on Saturday morning was an opportunity for people of all denominations and none, to gather for a short time to reflect with gratitude on her life and our thankfulness for her.'
Comhairle nan Eilean Siar Convener, Kenny Macleod, said: 'Our thoughts and prayers remain with the Royal Family at this sad time.
'The Queen visited the Western Isles on three official visits, in 1956, in 1979 to open the Comhairle nan Eilean Siar building and in 2002 for the Golden Jubilee. She also visited the Western Isles on many other occasions, with her family on cruises aboard the royal yacht Britannia.
'She had a very special place in the hearts of the people of these islands. Bidh sinn ga do chuimhneachadh.'
'Lord Lieutenant of the Western Isles, Iain Macaulay, said: 'HM The Queen has been a symbol of stability for the past 70 years as monarch, providing an outstanding example of service, dedication and Christian faith.
'During this extraordinary long reign, the world has seen many changes and her passing will have a significant impact in the UK, across the Commonwealth and throughout the world.
In the recent period of mourning and reflection, we acknowledged the strong and close connection between The Queen and her people. Communities across the Western Isles fondly remember with great pride The Queen’s visits to these islands.
We look back with gratitude and great respect for the Queen’s wonderful achievements in her long reign and offer our support and loyalty to King Charles III. Gad chuimhneachadh.'
For those unable to attend in person, the service can be found on the St Columba’s Church’s YouTube and Facebook pages.
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